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anti rabbit secondary antibody  (Jackson Immuno)


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    Jackson Immuno anti rabbit secondary antibody
    Anti Rabbit Secondary Antibody, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+sheep+antibody/bio_rxiv__64898__2026__03__24__713994-202-32-36?v=Jackson+Immuno
    Average 93 stars, based on 38 article reviews
    anti rabbit secondary antibody - by Bioz Stars, 2026-08
    93/100 stars

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    Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Journal: Frontiers in Veterinary Science

    Article Title: Optimizing ovine brucellosis serodiagnosis: evaluation of recombinant Brucella antigens and multi-antigen combinations for iELISA

    doi: 10.3389/fvets.2026.1775573

    Figure Lengend Snippet: Optimization of single-antigen iELISA conditions for recombinant Brucella antigens by checkerboard titration. For each antigen, coating concentration (left), serum dilution (middle), and HRP-conjugated secondary antibody dilution (right) were optimized using standard Brucella-positive and -negative reference sera. Data points represent mean OD450 ± SD (technical triplicates). Working conditions were selected based on the maximum P/N ratio. (A) rOmp19; (B) rOmp2b; (C) rOmp31; (D) rBP26.

    Article Snippet: Optimization parameters included: (i) coating concentration of the single antigen or antigen mixture, (ii) serum dilution, and (iii) working dilution of the HRP-conjugated secondary antibody (Rabbit anti-sheep IgG (H/L): HRP; BIO-RAD, 5184–2,504).

    Techniques: Recombinant, Titration, Concentration Assay